October 1586.

You have in various ways and manners attempted to take my life and to bring my kingdom to destruction by bloodshed. I have never proceeded so harshly against you, but have, on the contrary, protected and maintained you like myself. These treasons will be proved to you and all made manifest. Yet it is my will, that you answer the nobles and peers of the kingdom as if I were myself present. I therefore require, charge, and command that you make answer for I have been well informed of your arrogance.
Act plainly without reserve, and you will sooner be able to obtain favour of me.

ELIZABETH

Written at the opening of Mary Queen of Scots’ trial on the 12th of October 1586 at Fotheringhay Castle.

(Source: “The Letters of Queen Elizabeth I” edited by G B Harrison 1968)